MCCL (Monte Carlo Combinatorial Labeling) Program calculates the optimal labeling combination for a given protein sequence in order to use Cell-free combinatorial Dual Labeling (CDL) method for fast assignment of backbone 1H and 15N resonances.
The CDL method (described in Maslennikov et al., PNAS 2010) provides unambiguous assignment of the backbone resonances for 30-40% of the residues in a sequence and define the type of amino acid for backbone resonances for all residues.
MCCL program optimizes labeling scheme by using a minimal number of samples and by minimizing the spectral complexity of each sample.
The protein sequence is required for MCCL. Also please define below sets of 15N- and 13C-labeled amino acids and a number of NMR samples (typically 5 to 8 for 100-200 aa protein).
This is a free service for academic users. We will email you the MCCL results in 1-2 days after you submit your query online.
Please contact MCCL Team (firstname.lastname@example.org) if you have any questions regarding the CDL method or MCCL results.
Please contact Salk's Technology Transfer office (email@example.com) if you are interested in licensing the technology for industrial applications.